Figure 5

Confocal microscopy of MxA in infected cells. HeLa cells on coverslips were either left untreated or were pre-treated for 24 h with 2000 U/ml of IFN-β. After pre-treatment, the cells were infected with MPXV-Zaire at an MOI = 1 in the presence or absence of 2000 U/ml of IFN-β. Coverslips were harvested, fixed, permeabilized, and immunostained for MxA and A33 24 h p.i.. The coverslips were stained with Hoechst dye, mounted onto slides, and imaged by confocal microscopy. Green is A33, red is MxA, blue is Hoechst dye, and yellow represents the overlap of green and red fluorescence. Arrowheads point to the site of envelopment, arrows point to distinct areas of signal overlap, and asterisks denote the cell vertices.