Figure 3From: In vitro inhibition of monkeypox virus production and spread by Interferon-βHeLa fluorescence. HeLa cells were pre-treated for 24 h with the indicated concentrations of IFN-β. After pre-treatment, the cells were infected with MPXV-GFP-tdTR (MOI = 5) in the presence or absence of 2000 U/ml of IFN-β. The cells were imaged 24 h p.i. by fluorescence microscopy (left), and relative fluorescence was assayed using a fluorescence microplate reader (right). Green is GFP, red is Tomato Red, and yellow represents the overlap of green and red fluorescence.Back to article page