Table 1 The reverse transcription loop-mediated isothermal amplification (RT-LAMP) primer sets
Primer | Type | 5'pos | 3'pos | Length | Sequence(5' to -3') |
|---|---|---|---|---|---|
F3 | Forward outer | 519 | 539 | 21-nt | AAGGAACTATAGTATGGCGAA |
B3 | Backward outer | 707 | 724 | 18-nt | CTGTTGCTGGTCTCTTGT |
FIP | Forward inner | 592 | 616 | 46-mer(F1c:25-nt, | TGGACCTACAAATTGCACCAATATA- |
(F1c + F2) | 548 | 568 | F2:21-nt) | ACCTCTTACAGTTGCAAAGTG | |
BIP | Backward | 619 | 640 | 44-mer (B1c:22-nt, | AAGGACCCAGAGTGATGAGGTA- |
inner(B1c + B2) | 679 | 700 | B2:22-nt) | TGTATTTTCTTCCTTGGAACTT | |
LF | Loop Forward | 569 | 591 | 23-nt | CTCTTAGCTGCTAGTTCTGAAGC |
LB | Loop Backward | 641 | 663 | 23-nt | CCCTCGAAAGAATCTATTCAGGG |