Immune cells | CD3+ (T)cells | aCD4+T cells | aCD8+T cells | aCD4+CD8+ Th/memory |
|---|
Day-0
| 51.1 ± 11.1f | 21.4 ± 6.3 | 12.5 ± 3.5 | 23.0 ± 5.3 |
Day-2 contact
| 51.7 ± 15.8 | 18.9 ± 8.9 | 20.8 ± 10.3* | 21.0 ± 6.8 |
Day-2 infected
| 60.7 ± 15.2 | 17.9 ± 5.9 | 7.8 ± 2.3#, $ | 11.0 ± 3.5#, $ |
Immune cells
|
b
NK cells
|
c
γδ T cells
|
d
Myeloid cells
|
e
Tregs
|
Day-0
| 20.0 ± 11.2 | 25.6 ± 9.7 | 27.4 ± 6.1 | 0.2 ± 0.1 |
Day-2 contact
| 38.0 ± 23.9* | 15.6 ± 7.9* | 18.5 ± 12.8* | 1.0 ± 0.6* |
Day-2 infected
| 31.1 ± 18.7 | 27.2 ± 7.2$ | 11.3 ± 8.9#, $ | 1.1 ± 0.4# |
- aCD3+ and bCD3-cells were gated to enumerate CD4 and CD8α expression and grouped as follows: total T lymphocytes (CD3+ T cells); CD4+ T cells (CD3+CD4+CD8α-); CD8+ T cells (CD3+CD4-CD8α+); Th/memory cells (CD3+CD4+CD8α+); NK cells rich fraction (CD3-CD4-CD8α+). Other immune cells: c γδ T cells (CD8α+TcR1N4+); dMyeloid cells (CD172+). eCD25+ cells were gated to enumerate CD4 and Foxp3 expressing cells and the percent of total CD4+CD25+Foxp3+ cells are shown. PBMCs of all the seven contact viremic pigs were used in the study. fEach number is an average percent of immune cells from 7 to 13 pigs +/-standard deviation. Symbols indicate statistically significant difference (P < 0.05) between: *-day 0 and day 2 contact; #-day 0 and day 2 infected; $-day 2 contact and day 2 infected pig groups, analyzed by Wilcoxon t-test.
