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Figure 5 | Virology Journal

Figure 5

From: Heat shock protein 70 selectively mediates the degradation of cytosolic PrPs and restores the cytosolic PrP-induced cytotoxicity via a molecular interaction

Figure 5

Hsp70 proteins are up-regulated in HEK293 cells expressing Cyto-PrP and in the brains of scrapie-infected hamsters. A. Western blots of PrP and Hsp70 in cells expressing Cyto-PrP treated with or without MG132 (10 μM) for 4 h. The cell lysates were separated by 12% SDS-PAGE, transferred onto a nitrocellulose membrane and analyzed by immunoblotting with antibodies against PrP, Hsp70 or actin. B. Quantification of the immunoblots. The numerical gray values for PrP or Hsp70 were quantified and normalized to β-actin levels. All experiments presented in the figures were repeated independently 3 times with consistent results. Each data point represents the mean ± SEM of 3 independent experiments. C. Western blots of PrP and Hsp70 in the brain tissues of normal and scrapie 263K-infected hamsters. Ten percent of the brain homogenates from each animal was separated by 12% SDS-PAGE and blotted with antibodies against PrP, Hsp70 and actin. D. The numerical gray values for PrP or Hsp70 were quantified and normalized to β-actin levels. Each data point represents the mean ± SEM of three animals each group (n = 3).

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Virology Journal

ISSN: 1743-422X