Figure 4

(a) N RNA complex of PPRV was purified from virus infected vero cells . Coomassie stained gel of purified PPR N RNA complex, lane 1 marker, lane 2 N-RNA. (b) Presence of N protein was confirmed by western blot using antibody against recombinant N protein of PPRV (1:4000) [19]. (c) 20 μg of partially purified recombinant (L-P) complex was subjected to western blot analysis for P protein with polyclonal antibody to recombinant P protein (1:4000) [7]. (d) To confirm L protein presence in partially purified L-P complex, 300 μg of sample was immunoprecipitated using anti P antibody and western blotting was done using an antibody against domain III of bacterially expressed L protein of PPRV (1:5000). The bottom band in the panel d represents the heavy chain of immunoglobulin used in pull down experiment.