Figure 4
From: Permissiveness of human hepatoma cell lines for HCV infection
HCV sub-genomic and full-length replicon RNA replication in PLC and Hep3B cells. (A) Huh7, PLC and Hep3B cells were transfected with 1 μg of in vitro-transcribed sg JFH-1 RNA via electroporation. Cells were diluted 1:500 and plated in 100 mm tissue culture dishes and maintained in the presence of 500 μg/ml G418 for 2 weeks. Colonies were fixed, stained with crystal violet and counted. (B) Huh7, PLC and Hep3B cells were infected with 100 FFU of HCVccG418and maintained in the presence of 500 μg/ml G418 for 2 weeks. Colonies were fixed, stained with crystal violet and counted. (C-D) HCV G418-resistant sg and fl replicon clones were expanded in the presence of 500 μg/ml G418 and total RNA extracted ~3 weeks post expansion. HCV RNA was quantified by RTqPCR, normalized to GAPDH and is displayed as mean HCV RNA copies/μg total cellular RNA ± SD for each clone.