Figure 3

Transgene expression and inflammatory response after intramural injection of Ad5 and Ad5/35 vectors. Panels represent transgene expression and tissue damage after injection of Ad5-lacZ injection (A-C), Ad5/35-lacZ injection (D-F) or PBS (G-I). In panel C, a higher magnification image of B shows strong interstitial inflammatory cell infiltration, interstitial fibrosis and myocyte necrosis. Panel F, a higher magnification image of E, reveals weaker inflammation response and absence of myocyte damage. A mild needle tract reaction is seen in PBS-injected hearts (H and I). The sections were HE-stained and panels A, D and G show a light microscopic view from the same myocardial region stained with X-gal to visualize transgene expression. The scale bar in panel F represents 200 μm (for A-B, D-E and G-H) and 25 μm (for C, F and I).