Effect of CSFV infection on NF-κB translocation in PK-15 cells. A: Indirect immunofluorescence analysis was used to measure cellular localization of p65/RelA subunit of NF-κB in CSFV-infected PK-15 cells. Cells were mock treated (column 1), and TNF-α (10ng/mL) stimulated (column 4), or infected with Thiverval (column 2) and GXW-07 (column 3) at an MOI of 1. At different periods of time p.i, cells were fixed and the localization of p65/RelA (red) was observed by fluorescence microscope using immunofluorescence staining with anti-NF-κB/p65 and QDs-SA 605-conjugated and biotinylated secondary antibodies. B: Co-localization of p65/RelA (red) and IκBα (green) was observed by confocal microscopy. C: Nuclei were stained with DAPI. Bar, 10μm. The experiment was repeated three times and the figure shows a representative experiment.