Identification of the N-NS4B/WARF4 COOH-terminal amino acids sequence detected by MAb 3A12. The amino acids sequence of the N-NS4B/WARF4 COOH-terminal region with the graphical representation of the four synthetic peptides (SP1, SP2, SP3, SP4) covering the full amino acids sequence codified by WARF4 is shown on the top. The amino acids sequence of the four synthetic peptides is showed in the center, the overlapping sequences among the contiguous peptides are underlined. Peptides (500 ng), Envelope protein and BSA (500 ng) and His-WARF4 and His-NS4B proteins (50 and 150 ng, respectively) were analyzed by dot blotting employing MAb 3A12 (panel A), anti-NS4B antibody (panel B) and MAb anti-His (panel C). The star shows the peptide (SP2) recognized by MAb 3A12.