The development and application of new crystallization method for tobacco mosaic virus coat protein

Table 2 Protein building blocks used or referenced herein

Building block	Origin	Abbreviation
Wild type TMV coat protein	N. tabacum K₃₂₆	WT-TMV-CP^a
Wild type TMV coat protein with GST tags	E. coliBL ₂₁ (DE ₃ )-RIL	WT-GST-TMV-CP₃₂^b
Wild type TMV coat protein with His tags	E. coliBL ₂₁ (DE ₃ )-RIL	WT-His-TMV-CP₁₂^c
Truncated 19 TMV coat protein with His tags	E. coliBL ₂₁ (DE ₃ )-RIL	TR-His-TMV-CP₁₉^d
Truncated 62 TMV coat protein with His tags	E. coliBL ₂₁ (DE ₃ )-RIL	TR-His-TMV-CP₆₂^e
Truncated 68 TMV coat protein with His tags	E. coliBL ₂₁ (DE ₃ )-RIL	TR-His-TMV-CP₆₈^f

^aThe wild type TMV-CP was purified from N. tabacum K₃₂₆.
^bThe wild type TMV-CP expressed and purified from reconstructed prokaryotic expression vector PGEX-6P-1-TMV-CP₃₂ that was expressed in competent cell BL₂₁(DE₃)-RIL.
^cThe wild type TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP₁₂ that was expressed in competent cell BL₂₁(DE₃)-RIL.
^dThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP₁₉ that was expressed in competent cell BL₂₁(DE₃)-RIL_.
^eThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP₆₂ that was expressed in competent cell BL₂₁(DE₃)-RIL_.
^fThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP₆₈ that was expressed in competent cell BL₂₁(DE₃)-RIL.

ISSN: 1743-422X