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Table 5 Comparison of HA and NTA method

From: Evaluation of nanoparticle tracking analysis for total virus particle determination

   HA   NTA  
  V (ml) HA (HA/ml) Total HA (HA) Recovery (%) NTA (VP/ml) Total titer (VP) Recovery (%)
Load 1,5 128 192,0   5,7E+09 8,6E+09  
1 FT 2,9 <1    4,1E+08 1,2E+09 13,8
1 E1 1 128 128,0 66,7 5,8E+09 5,8E+09 68,0
1 E2 1,15 4 4,6 2,4 1,5E+08 1,7E+08 2,0
Total recovery (%)     69,1    83,0
2 FT 2,4 <1    6,0E+08 1,4E+09 16,7
2 E1 0,9 128 115,2 60,0 7,2E+09 6,5E+09 75,4
2 E2 1,1 4 4,4 2,3 3,7E+08 4,1E+08 4,7
Total recovery (%)     62,3    96,8
  1. Analysis of chromatographic samples collected during influenza virus purification on two different CIM SO3 monolithic columns by HA and NTA method; FT (flow-through fraction) and E (elution fractions).