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Table 2 Inter-day variability of the NTA method

From: Evaluation of nanoparticle tracking analysis for total virus particle determination

Sample

Dilution

Measured conc. (VP/ml)

Average measured conc (VP/ml)

Sample conc. (VP/ml)

MF

TF

TF/MF

Conc. (VP/ml)

Day 1

20

6,90E+08

      

20

4,27E+08

5,59E+08

1,12E+10

    

30

4,63E+08

  

1,3

1,5

1,1

1,2E+10

30

3,73E+08

4,18E+08

1,25E+10

    

Day 2

20

6,08E+08

      

20

8,12E+08

7,10E+08

1,42E+10

    

30

4,89E+08

  

1,4

1,5

1,1

1,5E+10

30

5,49E+08

5,19E+08

1,56E+10

    

Day 3

20

6,28E+08

      

20

6,75E+08

6,52E+08

1,30E+10

    

30

5,50E+08

  

1,3

1,5

1,2

1,4E+10

30

4,60E+08

5,05E+08

1,52E+10

    

Day 4

20

5,73E+08

      

20

5,35E+08

5,54E+08

1,11E+10

    

30

3,79E+08

  

1,5

1,5

1,0

1,1E+10

30

3,52E+08

3,66E+08

1,10E+10

    

Average

1,3E+10

STDV

1,8E+09

%STDV

14,1

  1. Inter-day variability of the NTA method determined by measuring the same sample (Ad5 virus) for 4 consecutive days. MF- the ratio between concentrations of two sample dilutions determined with NTA; TF-the ratio between two dilutions of the same sample analyzed.