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Table 2 Inter-day variability of the NTA method

From: Evaluation of nanoparticle tracking analysis for total virus particle determination

Sample Dilution Measured conc. (VP/ml) Average measured conc (VP/ml) Sample conc. (VP/ml) MF TF TF/MF Conc. (VP/ml)
Day 1 20 6,90E+08       
20 4,27E+08 5,59E+08 1,12E+10     
30 4,63E+08    1,3 1,5 1,1 1,2E+10
30 3,73E+08 4,18E+08 1,25E+10     
Day 2 20 6,08E+08       
20 8,12E+08 7,10E+08 1,42E+10     
30 4,89E+08    1,4 1,5 1,1 1,5E+10
30 5,49E+08 5,19E+08 1,56E+10     
Day 3 20 6,28E+08       
20 6,75E+08 6,52E+08 1,30E+10     
30 5,50E+08    1,3 1,5 1,2 1,4E+10
30 4,60E+08 5,05E+08 1,52E+10     
Day 4 20 5,73E+08       
20 5,35E+08 5,54E+08 1,11E+10     
30 3,79E+08    1,5 1,5 1,0 1,1E+10
30 3,52E+08 3,66E+08 1,10E+10     
Average 1,3E+10
STDV 1,8E+09
%STDV 14,1
  1. Inter-day variability of the NTA method determined by measuring the same sample (Ad5 virus) for 4 consecutive days. MF- the ratio between concentrations of two sample dilutions determined with NTA; TF-the ratio between two dilutions of the same sample analyzed.