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Table 1 Summary statistics for targeted viruses and sequencing results

From: Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

Virus type

Forward success rate (%)

Reverse success rate (%)

Average success rate (%)

Number of reactions successful

Number of reactions total

Ambiguity in consensus (%)

GC content of consensus sequence (%)

Number of sequences in consensus

Number of amplicons designed

Minimum coverage

BioProject ID

HPIV-1

98.95

100.00

99.47

378

380

5.10

34.64

5

95

3x

PRJNA73053

HPIV-3

98.42

97.89

98.16

373

380

7.77

31.24

5

95

3x

PRJNA73055

MeV

93.41

95.05

94.23

1738

1860

10.76

41.94

25

91

3x

PRJNA69913

MuV

73.85

73.68

73.77

1963

2661

9.28

37.97

32

96

3x

PRJNA73019

RUBV-G1

80.05

78.63

79.34

3556

4882

6.07

66.75

11

96

2x

PRJNA70479

RUBV-G2

71.52

69.24

70.38

1478

2100

13.13

61.37

5

96

2x

PRJNA70479

HRSV-A

91.32

89.96

90.64

3004

3332

6.81

30.25

3

50

2x

PRJNA73049

HRSV-B

91.37

90.86

91.11

2436

2682

4.12

31.79

3

59

2x

PRJNA73049

HMPV-A

90.99

88.82

89.91

1737

1932

9.25

31.53

6

42

1x

PRJNA73051

HMPV-B

86.16

87.04

86.60

788

910

7.95

32.68

5

36

1x

PRJNA73051