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Table 1 Summary statistics for targeted viruses and sequencing results

From: Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

Virus type Forward success rate (%) Reverse success rate (%) Average success rate (%) Number of reactions successful Number of reactions total Ambiguity in consensus (%) GC content of consensus sequence (%) Number of sequences in consensus Number of amplicons designed Minimum coverage BioProject ID
HPIV-1 98.95 100.00 99.47 378 380 5.10 34.64 5 95 3x PRJNA73053
HPIV-3 98.42 97.89 98.16 373 380 7.77 31.24 5 95 3x PRJNA73055
MeV 93.41 95.05 94.23 1738 1860 10.76 41.94 25 91 3x PRJNA69913
MuV 73.85 73.68 73.77 1963 2661 9.28 37.97 32 96 3x PRJNA73019
RUBV-G1 80.05 78.63 79.34 3556 4882 6.07 66.75 11 96 2x PRJNA70479
RUBV-G2 71.52 69.24 70.38 1478 2100 13.13 61.37 5 96 2x PRJNA70479
HRSV-A 91.32 89.96 90.64 3004 3332 6.81 30.25 3 50 2x PRJNA73049
HRSV-B 91.37 90.86 91.11 2436 2682 4.12 31.79 3 59 2x PRJNA73049
HMPV-A 90.99 88.82 89.91 1737 1932 9.25 31.53 6 42 1x PRJNA73051
HMPV-B 86.16 87.04 86.60 788 910 7.95 32.68 5 36 1x PRJNA73051