Fluorescent images of Vero cells infected with E7-rRSV-X over time. Initial input is 115 PFU per well. The images show plaques produced by RSV expressing EGFP. At the indicated time points, plaques were detected after 27 hrs, 48 hrs and 72 hrs. in a fluorescence Elispot reader, manual counted (upper panel) and counted using the AID EliSpot Software 'algorithm C' with emphasis settings were set on ”big” (middle panel) or “tiny” (lower panel). Each yellow ‘x’ represents one plaque counted by the imager software.