Whole genome sequencing of phage resistant Bacillus anthracismutants reveals an essential role for cell surface anchoring protein CsaB in phage AP50c adsorption

Table 3 Results of Sanger verification of the various spontaneous AP50 ^R mutants

Sanger run ID	Strain	Sanger data	Mutation	454 data	Implication	BAS3946
csaB 1	34F₂ parent	WT	None	WT	-	G1024A
csaB 2	S-R1	∇ G between positions 46 and 47	Frame-shift	Same	Truncation of CsaB	G1024A
csaB 3	S-R2	∇ G between positions 233 and 234	Frame-shift	Same	Truncation of CsaB	G1024A
csaB 4	S-R3	∇ G between positions 233 and 234	Frame-shift	Same	Truncation of CsaB	G1024A
csaB 5	S-R4	Δ A at position 203	Frame-shift	Same	Truncation of CsaB	G1024A
csaB 6	S-R5	C385T	Nonsense	ND	Truncation of CsaB	G1024A
csaB 7	S-R6	∇ GCTTA between position 219-220	Frame-shift	Same	Truncation of CsaB	G1024A
csaB 8	S-R7	T274G	Y92D	ND	Noncharged to charged (−)	G1024A
csaB 9	S-R8	Δ C at position 129	Frame-shift	ND	Truncation of CsaB	WT
csaB 10	S-R9	C254 T	T85M	ND	Polar hydrophilic to nonpolar hydrophobic	WT
csaB 11	JB220 parent	WT	None	WT	-	WT
csaB 12	J-R1	G at position 573 to T	R191S	Same	Charged (+) to noncharged	WT
csaB 13	J-R2	∇ G between positions 233 and 234	Frame-shift	ND	Truncation of CsaB	WT
csaB 14	J-R3	G421T	Nonsense	ND	Truncation of CsaB	WT
csaB 15	J-R4	C 292T	Nonsense	ND	Truncation of CsaB	WT
csaB 16	J-R5	G796C	G266R	ND	Noncharged to charged (+)	WT
csaB 17	J-R6	T258G	S86R	ND	Noncharged to charged (+)	WT
csaB 18	J-R7	∇ G between positions 233 and 234	Frame-shift	ND	Truncation of CsaB	WT
csaB 19	J-R8	C767A	Nonsense	ND	Truncation of CsaB	WT

∇ and Δ indicate insertion and deletion of base(s) respectively at the indicated position from the start of the gene. ND- Not Done.
WT- Wild type; G1024A indicates a SNP in BAS3946 at position 1024 from the start of gene from G to A.

ISSN: 1743-422X