Primer pair | Sequence (5'-3') | Target region | Amplicon size (bp) |
---|---|---|---|
LR3.HRM1.F | TAGACGTTAAAGATGTGAAGCG | GLRaV-3 ORF1a | 167 |
LR3.HRM1.R | TCGTACACATCCACCATA | Â | Â |
LR3.HRM2.F | GTCCTAGATTCGGATTTTGTCG | GLRaV-3 ORF1a | 231 |
LR3.HRM2.R | GAATACTCTTCGCCCTATC | Â | Â |
LR3.HRM3.F | CTGGTTGCTTTCGAGGTATATGAG | GLRaV-3 ORF1b | 295 |
LR3.HRM3.R | CACTTCAAGGTGTTGCGCTT | Â | Â |
LR3.HRM4.F | TAATCGGAGGTTTAGGTTCC | GLRaV-3 ORF4 | 226 |
LR3.HRM4.R | GTCGGTTCGTTAACAACAC | Â | Â |
LR3.HRM5.F | TGTGTAAGAAGGTTATGGG | GLRaV-3 ORF6 | 224 |
LR3.HRM5.R | TACTGCCTTACCGGGTTTTC | Â | Â |
LR3.HRM6.F | GTCACCAGGTGTTCCAAACC | GLRaV-3 ORF1a | 305 |
LR3.HRM6.R | AACGCCCTGTATGTCCTCTC | Â | Â |
LR3_Universal_F | TAAATGCTCTAGTAGGATTC | GLRaV-3 5'UTR | Â |
621_430R | TAACCCAACACGACGATGAG | GLRaV-3 5'UTR | 429a |
623_564R | CTCACGCTAAACACACCAAG | GLRaV-3 5'UTR | 563a |
PL20_315R | GTTTGTAACAAAGAAACACG | GLRaV-3 5'UTR | 314a |
GH11_180R | CCAAAACGAAGACGAAAAGAAGAG | GLRaV-3 5'UTR | 179a |
LR_ORF1aR | CGTCCGCTTCACCCCTTTGG | GLRaV-3 ORF1a | Â |
Vv_Actin_F [32] | CTTGCATCCCTCAGCACCTT | V. vinifera predicted actin-7 | 82 |
Vv_Actin_R [32] | TCCTGTGGACAATGGATGGA | Â | Â |