Table 1 List of primers for quantitative RT-PCR
Reference | Gene | Primer | 5' modification | Sequence (5' to 3') | 3' modification |
|---|---|---|---|---|---|
Primer and probes used for the one-step qRT-PCR from TAKARA | |||||
NM_002201.4 | ISG20 | ISG20-636 F | CTGTTGTGGCGTGAGGCCA | ||
ISG20-764R | TGCCCTCGCATCTTCCACC | ||||
ISG20-688P | HEX | TGCTGAGTGAGCGCCTCCTGC | BHQ-1 | ||
NM_002201.4 | ISG54 | ISG54-76 F | GGTCTCTTCAGCATTTATTGGTG | ||
ISG54-219R | TGCCGTAGGCTGCTCTCCA | ||||
ISG54-124P | FAM | TGCAGCTGCCTGAACCGAGC | BHQ-1 | ||
NM_001548.3 | ISG56 | ISG56-131 F | GCCTAATTTACAGCAACCATGAG | ||
ISG56-347R | GGCCTTTCAGGTGTTTCACATA | ||||
ISG56-215P | HEX | TGGGAGTTATCCATTGATGACGATG | BHQ-1 | ||
NM_014314.3 | RIG-I | RIG-I-237 F | AGCTACATGGCCCCCTGGT | ||
RIG-I-397R | GCATGGTCTAGGGCATCCAA | ||||
RIG-I-351P | HEX | CAGGAGGAAGGCTGGTTCCGT | BHQ-1 | ||
NM_002046 | GAPDH | GAPDH-F | AGCCTCAAGATCATCAGCAATG | ||
GAPDH-R | CTGTGGTCATGAGTCCTTCCA | ||||
GAPDH-P | HEX | CCAACTGCTTAGCACCCCTGGC | BHQ1 | ||
Primers used for the RT-qPCR with BRYT Green | |||||
NM_002176.2 | IFN-β | F | GTTCGTGTTGTCAACATGACCA | ||
R | TCAATTGCCACAGGAGCTTCT | ||||
NM_016562.3 | TLR7 | F | CCACAACCAACTGACCACTG | ||
R | CCACCAGACAAACCACACAG | ||||
NM_002046.3 | GAPDH | F | GCACCGTCAAGGCTGAGAAC | ||
R | GCCTTCTCCATGGTGGTGAA | ||||