Figure 2

CHIKV modulates the expression of the IFN pathway and IFN-stimulated genes in both human fibroblast cell lines HS 633T and HT-1080. Relative expression of RIG-I, TLR7, IFN-β, ISG20, ISG54 and ISG56 from cells infected with CHIKV MOI of 1 for 8, 24, 48 h and mock-infected cell (CTL) in HS 633T (A) or HT-1080 (B) as assessed by real-time quantitative PCR. All experiments were done in triplicates and results are expressed as mean ± standard error (*: p < 0.05; **: p < 0.01; ***: p < 0.001).