Figure 1

Human fibroblast cell lines HS 633T and HT-1080 are differently susceptible to chikungunya virus (CHIKV) infection. (A) Immunostaining (polyclonal anti-CHIKV, green) of HS 633T and HT-1080 cells infected with CHIKV MOI of 1 (d, e, f and j, k, l respectively) or mock-infected (a, b, c and g, h, i respectively). Nuclei were stained with DAPI (blue), view X200. The experiments were performed in triplicate. (B) Percentage of HS 633T and HT-1080 CHIKV-infected (green staining) at a MOI of 1 at 48 h post-infection. Green fluorescent cells were counted in three observation fields and results are expressed as mean ± standard error. (C) Quantification of the viral load by real time qRT-PCR from HS 633T and HT-1080 supernatants infected with CHIKV MOI 1 for 8, 24 and 48 h. The experiment was done in duplicate and results are expressed as mean ± standard error.