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Table 1 Summary of morphological observations of VLPs under different storage conditions

From: Stability studies of HIV-1 Pr55gagvirus-like particles made in insect cells after storage in various formulation media

Storage time (months)
Formulation medium Temperature 1 3 6 12
PBS 4°C × nd nd
  -20°C ± × nd nd
  -70°C ± × nd nd
5% Trehalose 4°C × nd nd nd
  -20°C ± × nd nd
  -70°C × nd nd nd
15% Trehalose 4°C × nd nd
  -20°C
  -70°C
5% Sucrose 4°C × nd nd nd
  -20°C × nd nd nd
  -70°C × nd nd nd
15% Sucrose 4°C × nd nd
  -20°C ± × nd
  -70 × nd
5% Sorbitol 4°C nd nd nd nd
  -20°C ± × nd nd
  -70°C ± × nd nd
15% Sorbitol 4°C 4°C nd nd nd
  -20°C × nd nd
  70°C ± × nd
  1. √: the formulated VLP micrograph is identical to that of the fresh batch.
  2. ±: 50% or more of the formulated VLPs in the micrograph have changed shape.
  3. ×: 100% of the formulated VLPs have changed shape.
  4. nd: not determined.