Table 1 Oligonucleotides used for the generating the deletion mutants and other clones for the WSSV ie1 promoter activity assays
From: Sf-PHB2, A new transcription factor, Drives WSSV Ie1Gene Expression via a 12-bp DNA Element
plasmids | primers (5’ → 3’) |
|---|---|
p(-335/+53) | Fwd1 (CGGGTACC GTGGGTCTAAAACCGGGAGA) |
| Â | Rev1 (CCAAGCTTCTTGAGTGGAGAGAGAGA) |
p(-235/+53) | Fwd2 (CGGGTACC GACCATTCCCTAAGTTGAGGA)/Rev1 |
p(-135/+53) | Fwd3 (CGGGTACC CTTGCAGATGAAAATGGCTGT)/Rev1 |
p(-120/+53) | Fwd4 (CGGGTACC GGCTGTTTGAATCATGT)/Rev1 |
p(-105/+53) | Fwd5 (CGGGTACC GTTAAGGAATTTCCTTGT)/Rev1 |
p(-78/+53) | Fwd6 (CGGGTACC ATTCCTAGAAATGGTGT)/Rev1 |
p(-66/+53) | Fwd 7 (CGGGTACC GGTGTAATCGCTGTTGT)/Rev1 |
p(-35/+53) | Fwd 8 (CGGGTACC TGTGTATATAAGAGCCCGTGT)/Rev1 |
p(-55/+53) | Fwd 9 (CGGGTACC TGTTGTGGGCGGAGCATATTTGT)/Rev1 |
p(-335/+49) | Rev2(CCAAGCTT AGTGGAGAGAGAGAGCTAGT)/Fwd1 |
p(-335/+3) | Rev3(CCAAGCTT ACTGAATCGAGGAGCTAACA)/Fwd1 |
p(-335/-3) | Rev4 (CCAAGCTT ATCGAGGAGCTAACACGGGCT)/Fwd1 |
p(-35/+53Δ) | Mut1(TGTTGTGaGaGGAGCATATT)/Rev1/Fwd 8 |
p(-55/+53Δ) | Mut2(TGTGTATtaAAGAGCCCGTG)/Rev1/Fwd 9 |