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Table 1 Characteristics of the screening of EBV transformed B-cell cultures with V3C-CTB

From: Production and characterization of human anti-V3 monoclonal antibodies from the cells of HIV-1 infected Indian donors

1Number of HIV-1 infected patients

33

2 Total number of PBMCs isolated (million)

321.2

3 Number of wells plated

3321

4 Number of wells positive for V3-CTB

199

5 Number of anti-V3 antibody secreting cell lines

3

  1. 133 HIV-1 infected drug naïve patient donor samples were recruited for antibody production.
  2. 2A total of 321.2 million PBMCs were isolated from these 33 patients with a mean of 9.72 million per individual (range = 1.7 to 30 million).
  3. 3The PBMCs were plated in 96 well tissue culture plates @ 80-100 k cells/well and approximately 6% (199/3321) of the wells were positive with V3-CTB4.
  4. 5Three anti-V3 monoclonal antibody secreting clones 277, 903 and 904 were stabilized.
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Virology Journal

ISSN: 1743-422X