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Figure 2 | Virology Journal

Figure 2

From: Induction of interleukin-10 is dependent on p38 mitogen-activated protein kinase pathway in macrophages infected with porcine reproductive and respiratory syndrome virus

Figure 2

Viral replication was not obviously affected when cells were treated with inhibitors of p38 (SB203580), ERK1/2 (PD98059), PI3K (LY294002), and NF-κB (BAY11-7082) pathways. (a) BMDMs were pretreated with DMSO or PI3K inhibitor LY294002, ERK MAPK inhibitor PD98059, p38 inhibitor SB203580, and NF-κB inhibitor BAY11-7082 at the indicated concentrations for 2 h. Cells were then infected with PRRSV (MOI = 1) and harvested at 24 h.p.i.. Virus titers in the cell supernatants were measured by a standard 50% tissue culture infective doses (TCID50) assay. (b) BMDMs were treated with DMSO or PI3K inhibitor LY294002, ERK MAPK inhibitor PD98059, p38 inhibitor SB203580, and NF-κB inhibitor BAY11-7082 at the indicated concentrations for 24 h. Then the cytotoxicity of the inhibitors on BMDMs was determined by trypan blue exclusion dye staining. Data represent means ± SD of three independent experiments.

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