Table 2 Primer sequences used in this study
PCR analysis and DNA sequencing analysis | |||
|---|---|---|---|
Target gene | Primer name | Sequence (5’ → 3’) | Predicted product size |
MCPyV LT | LTsh-F | GATCAGGAGGATTCAGCTTCG | 242 bp |
| Â | LTsh-R | CAGAGGATGAGGTGGGTTCC | Â |
MCPyV VP1 | VP1-F | TTTGCCAGCTTACAGTGTGG | 352 bp |
| Â | VP1-R | TGGATCTAGGCCCTGATTTTT | Â |
HPV L1 | GP5+ | TTTGTTACTGTGGTAGATACTAC | 150 bp |
| Â | GP6+ | GAAAAATAAACTGTAAATCATATTC | Â |
Human β-globin | β-globin-F | ACACAACTGTGTTCACTAGC | 110 bp |
|  | β-globin-R | CAACTTCATCCACGTTCACC |  |
Real-time PCR analysis | Â | Â | |
Target gene | Primer sequence (5’ → 3’) | Probe sequence (5’ → 3’) | |
MCPyV ST | GCAAAAAAACTGTCTGACGTGG | FAM-TATCAGTGCTTTATTCTTTGGTTTGGATTTCCTCCT-TAMRA | |
| Â | CCACCAGTCAAAACTTTCCCA | Â | |
Human RNase P | AGATTTGGACCTGCGAGCG | FAM-TTCTGACCTGAAGGCTCTGCGCG-TAMRA | |
| Â | GAGCGGCTGTCTCCACAAGT | Â | |