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Table 1 Oligonucleotide primers used in this study

From: Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

primer

Sequence

FΔ480-532

5′- TCCCTAACGGTTCGGAAGAAACAACGCTGACGCACCAGGA -3′

RΔ480-532

5′- TCCTGGTGCGTCAGCGTTGTTTCTTCCGAACCGTTAGGGA -3′

FΔ508-532

5′- AGCCCGTACTTATGCCCGCGACAACGCTGACGCACCAGGA -3′

RΔ508-532

5′- TCCTGGTGCGTCAGCGTTGTCGCGGGCATAAGTACGGGCT -3′

FΔ480-620

5′-TCCCTAACGGTTCGGAAGAAGTGTCATCAAGCAGCCCCCT-3

RΔ480-620

5′-AGGGGGCTGCTTGATGACACTTCTTCCGAACCGTTAGGGA-3

FΔ480-667

5′-TCCCTAACGGTTCGGAAGAATGTGATGCGTCCAAGCTTGG-3′

RΔ480-667

5′-CCAAGCTTGGACGCATCACATTCTTCCGAACCGTTAGGGA-3′

FΔ444-667

5′-CTCGAAGAACAAAGTCTGTCTGTGATGCGTCCAAGCTTGG-3′

RΔ444-667

5′-CCAAGCTTGGACGCATCACAGACAGACTTTGTTCTTCGAG-3′

FΔ420-532

5′-AGCAGGTCAATTTAAAAGCTACAACGCTGACGCACCAGGA-3′

RΔ420-532

5′-TCCTGGTGCGTCAGCGTTGTAGCTTTTAAATTGACCTGCT-3′

FΔ400-532

5′-AGGATCTGCTAAAACTAGCCACAACGCTGACGCACCAGGA-3′

RΔ400-532

5′-TCCTGGTGCGTCAGCGTTGTGGCTAGTTTTAGCAGATCCT-3′

FΔ400-700

5′-AGGATCTGCTAAAACTAGCCCAGGCGTTTCGCATCTTAAG-3′

RΔ400-700

5′-CTTAAGATGCGAAACGCCTGGGCTAGTTTTAGCAGATCCT-3′

F2436

5′-ATATGGGCTCATGTCCACTG- 3′

R3442

5′-GTAACATCACAAACCCGCAC- 3′

F14320

5′- CACAGCTCCACAGAAGGTGC-3′

R14752

5′- TAACAGCTTTTCTGCCACCC-3′