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Figure 3 | Virology Journal

Figure 3

From: Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

Figure 3

Identification of the rescued viruses by RT-PCR. One pair of primers (F2436/R3442) amplifying deletion region of the nsp2 was used to confirm the presence of deletions (A). Another pair of primers (R14320/F14752) amplifying part of PRRSV was used to identify the recombinant viruses containing a Mlu I enzyme site as gene marker, The results of RT-PCR products digested by Mlu I indicated that the recombinant viruses were rescued from cDNA clones (B).

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Virology Journal

ISSN: 1743-422X