Analysis of inflammatory cytokine production following immunization with baculoviruses. Real-time PCR was conducted for three cytokines, TNF-α, IL-2 and IL-6. Total RNA was obtained as described in Figure 4. The graphs obtained for the expression of TNF-α gene, IL-2 gene and IL-6 gene, respectively. Data are presented as means ± SD (n = 3). *, p<0.05; **, p<0.01 compared to the value for 109 PFU of Bac-G-EGFP (control virus)-immunized group.