Table 1 Sequences of primers used in the modified nPCR method

First PCR

 BF

5’- ACG GGG CGC ACC TCT CTT TA -3’

1 519–1 538

 HBAS-4 V

5’- ATA GGG GCA TTT GGT GGT CT -3’

2 316–2 297

 PF

5’- TTA TGC CTG CTA GGT TY^aT ATC C -3’

2 635–2 656

 S4R

5’- AGA AGA TGA GGC ATA GCA GC -3’

434-415

Second PCR

Mix A

 HB

5’- ACC GTG AAC GCC CAC M^bGG AA -3’

1 617–1 636

 BJA-RV

5’- TTC TTT ATA CGG GTC AAT GTC CAT G -3’

1 924–1 900

 DF

5’- GCA GAA TCT TTC CAC CAG -3’

2 853–2 870

 C1F

5’- TCA CTC CR^cC CAC ACG GCA A -3’

3 047–3 065

 C2F

5’- CAC CGA ACA TGG AGA R^cCA CA -3’

147-166

 PR

5’- TTG GTG AGT GAT TGG AGG TTG -3’

341-321

Mix B

 AF1

5’- GCC TAC TAG ATT CTA TCC TAC CCA C -3’

2 645–2 669

 AF2

5’- GCC TAC TAG ATT TTA TCC TAA CAG C -3’

2 645–2 669

 BA1R

5’- CTC GCG GAG ATT GAC GAG ATG T -3’

111-132

Mix C

 HB

5’- ACC GTG AAC GCC CAC M^bGG AA -3’

1 617–1 636

 BA

5’- GTG TCG AGR^c AGA TCT CGA ATA -3’

1 998–1 978

 BJ

5’- TGA TCT TTA GGC CCA TGT TAG T -3’

2 192–2 171