Figure 3
From: Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1
Growth curves of wtNDV/9a5b-D5C1 and rNDV/9a5b-D5C1 in DF1 cells. Cell monolayers with 80 % confluence in a 6-well plate were transfected with wtNDV/9a5b-D5C1 and rNDV/9a5b-D5C1 at a multiplicity of infection (MOI) of 0.01, and the supernatant was harvested at 2, 4, 6, 8, 12, 16, 24, 36, and 48 h post-infection. The 50 % tissue culture infection dose (TCID50) of the virus was determined to quantify the live viruses secreted into the supernatant at each time point. All titrations were performed in triplicate. Bars show standard deviations.