MAb: | MHIAF | 4G2 | 6B6C-1 | 4A1B-9 | 23-1 | 23-2 | 20 | 5-1 | 5-2 | 1B7-5 | D3-5 C9-1 | 1A1D-2 | 9D12 | 10A4D-2 | 1B4C-2 | 3 H5 |
|---|
CR2: | Poly-clonal | group | group | group | group | group | Sub grp. | Sub grp. | Sub grp. | comp. | comp. | Sub comp. | Sub comp. | Sub comp. | Sub comp. | Type-spec. |
|---|
Virus:
| D2 | D2 | SLEV | MVEV | WNV | JEV | JEV | D2 | JEV | D3 | D4 | D2 | D1 | D2 | D2 | D2 |
VLP construct
|
WT DENV-23 | ≥6.0 | ≥6.0 | ≥6.0 | 5.1 | ≥6.0 | ≥6.0 | ≥6.0 | ≥6.0 | ≥6.0 | ≥6.0 | 5.1 | ≥6.0 | ≥6.0 | ≥6.0 | >4.2 | ≥6 |
RD5 | 100 |
<0.1
|
<0.1
|
<3
|
0.2
|
0.2
| 100 |
3
| 100 | 100 | 25 | 100 | 100 |
6
| nd4 | 50 |
ERR | 100 | 100 | 100 | 150 | 100 | 100 |
<0.1
|
<0.1
| 100 | 100 | 100 |
0.2
|
0.1
| 100 | nd | 100 |
RDERR | 100 |
<0.1
|
0.2
|
<0.8
|
<0.1
|
0.2
|
<0.1
|
<0.1
| 100 | 100 |
1.5
|
0.1
|
0.4
| 100 |
<1.3
| 100 |
- 1Percent of wild-type (WT) DENV-2 VLP reactivity for MAbs exhibiting varying levels of cross-reactivity (CR) and selected from different flaviviruses.
- 2 MHIAF is polyclonal murine ascitic fluid; group CR antibodies recognize all viruses of the four major pathogenic flavivirus serocomplexes; sub-group CR MAbs recognize all or some members of two or more different flavivirus serocomplexes (e.g., MAbs 20, 5–1 and 5–2 recognize DENV-2 and JEV, JEV and DENV-2 and JEV, DENV-1 and −2 respectively); comp. and sub-comp. CR MAbs recognize all four DENV complex viruses or a subset thereof respectively, and type-specific MAbs recognize only DENV-2.
- 3 MAb reactivities for wild-type (WT) DENV-2 VLP are presented as inverse log10 antigen-capture ELISA endpoint values and all mutant VLPs as percent of remaining reactivity compared to WT.
- 4 nd denotes not determined.
- 5Mutant VLPs containing amino acid substitutions in the EDIIFP G106R/L107D (RD), in EDIII K310E/E311R/P364R (ERR), or in both the EDIIFP and EDIII G106R/L107D/K310E/E311R/P364R (RDERR).
- Reproduced in part from: Crill WD, Hughes HR, Delorey MJ, Chang GJ (2009) Humoral immune responses of dengue fever patients using epitope-specific serotype-2 virus-like particle antigens. PLoS ONE 4: e4991.
