Loop-mediated isothermal amplification for the detection of goose circovirus

Table 1 Sequences of LAMP primers used in the study

Primer	Sequence 5’ – 3’	Length (nt)	Localisation in GCV genome
F3	CTGCGGTGTCTTCTGCTT	18	1080-1097
B3	AGTGGAACGTAGACCCCTAC	20	1294-1313
BIP (B1c + B2)	TGTTGGATGGTTGGTTTCGGGATTTT GAAAACGTGGTCACCTCACA	46	1214-1235, 1257-1276
FIP (F1c + F2)	ACACCCCAGGAGGAAGACAACTTTTT TAGTGACATGCCCATTCCGT	46	1157-1178, 1102-1121
LF	ATTCAAGACAACGTAGTATTCT	22	1122-1143
LB	CAAATACTCTTTTGTGGTATC	21	1236-1256

F3 – forward outer primer, B3 – forward backward primer, FIP – forward inner primer (F1c + F2), BIP – backward inner primer (B1c + B2), LF – forward loop primer, LB – backward loop primer, TTTT – thymine linker.

ISSN: 1743-422X