Figure 4
From: Development of persistent HCV genotype 3a infection cell culture model in huh-7 cell
Inhibitory effect of HCV-3a core gene specific siRNA in Huh-7 infectious model. (A) Real time Real time PCR of core gene expression level after siRNA (Csi476) transfection in HCV genotype 3a serum infected Huh-7 cells at different days (D1 to D5). Maximum inhibition (92%) was seen at Day 1 of post infection. Core gene expression started to gradually increase till 5th day. Huh-7 cells infected with HCV genotype 3a serum (H + S3a) and scramble siRNA (Sc) used as positive control. Three independent experiments were performed having triplicate samples. Error bars indicate SD, *p < 0.01 GAPDH was used as internal control. (B) Western blot of HCV Core protein in HCV genotype 3a serum infected Huh-7 cells transfected with Csi476 from day one (D1) 1 to day five (D5). Maximum HCV core protein inhibition was seen on day 1 (D1) as compared to Huh-7 cells infected with HCV genotype 3a serum (H + S3a) and scramble siRNA (Sc) that were used as positive control. Huh-7 cells were used as negative control and GAPDH was used as internal control.