Figure 4From: The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA Presence of 18s rRNAs and tRNAs in immunoprecipitated complexes of Nef and RPS10. 18s rRNA and tRNAs are present in Nef (A) and RPS10 (B) co-immunoprecipitated complexes. MDMs were treated with rNef (100 ng/ml) for 2 h or left untreated (mock). Total cellular extracts were prepared and the detection of tRNAMet, tRNATryp, tRNAPhe, tRNALys3, tRNAyeast (as a negative control) and 18S rRNA was performed in lysates immunoprecipitated with Nef and RPS10 antibodies respectively, followed by RNA extraction and qRT-PCR amplification as previously described [28]. Results are representative of two independent experiments.Back to article page