Figure 1

IgG ELISA and immunoblot responses following experimental infection by sc and ivg routes. Panel 1A, Pattern of seroconversion following inoculation via sc (left panel) and ivg (right panel) routes assessed by ELISA. Data are expressed as reciprocal of endpoint dilution titer based on limiting dilution ELISA assay as described in the text. Mean +/- SD is shown for each time point. IgG responses trended higher in non-pregnant compared to pregnant animals but no statistically significant differences were noted. Within both groups, sc inoculation elicited a higher IgG titer at week 8 post-inoculation (p < 0.01) and week 12 post-inoculation (p < 0.001) by ANOVA. Panel 1B, representative western blot analysis of temporal sequence of antibody responses in non-pregnant animal inoculated by sc route (left side of figure) and ivg route (right side of figure).