Table 2 Primer sequences used in plasmid construction
From: A rapid method to screen putative mRNA targets of any known microRNA
Genes inserted | Sequences |
|---|---|
MiR-UL112-1 | F: 5'-CGCGGATCC TCAGGTACTCGCAGGTGTGC |
| Â | R: 5'-CCCAAGCTT GTTGCCTGGACGCCTGGGCGCGA |
HCMV IE72 | F:5'-GGACTAGT ACTATTGTATATATATATCAGT |
| Â | R:5'-CCCAAGCTT CGGTTTCACAGGCGTGACACGTT |
Homo sapiens zinc finger protein 36, C3H type-like 1 (ZFP36L1) | F:5'-GGACTAGT AGGCCTTTCACAACTAGGACTGA |
| Â | R:5'-CCCAAGCTT AAACTGCAAATAGTCGTTACAAA |
Homo sapiens transportin 1 | F:5'-GGACTAGT TCTAATACACTTAAGCTGCAGT |
| Â | R:5'-CCCAAGCTT GCTTCTTCACATCCACTGCGGAGT |
Homo sapiens ribosomal protein L7a | F:5'-GGACTAGT GAAGACAAAGGCGCTTTGGCTA |
| Â | R:5'-CCCAAGCTT ATGTACAGAAAACTCAACAGT |
Homo sapiens interleukin 32 | F:5'-GGACTAGT AGATACTGACACCACCTTTGCCCT |
| Â | R:5'-CCCAAGCTT CATGGTATCTCCCCTGCCAG |
Homo sapiens mRNA for putative NFkB activating protein | F:5'-GGACTAGT TGAACACAGAAAGTCTAAGAGGA |
| Â | R:5'-CCCAAGCTT GCTAATTAAACTTTGATTTTATTATG |
HCMV UL17/18 | F:5'-GGACTAGT TACCAGCGGTTACGCACCGAG |
| Â | R:5'-CCCAAGCTT AACAGTTCCTCGGACATGATCA |
HCMV IE86 | F:5'-GGACTAGT AGTCCACGGACCGCTCGGTCT |
| Â | R:5'-CCCAAGCTT TGCGCTCACCCGGCGTTCTC |