Table 2 Primer sequences used in plasmid construction
From: A rapid method to screen putative mRNA targets of any known microRNA
| Genes inserted | Sequences |
|---|---|
| MiR-UL112-1 | F: 5'-CGCGGATCC TCAGGTACTCGCAGGTGTGC |
| R: 5'-CCCAAGCTT GTTGCCTGGACGCCTGGGCGCGA | |
| HCMV IE72 | F:5'-GGACTAGT ACTATTGTATATATATATCAGT |
| R:5'-CCCAAGCTT CGGTTTCACAGGCGTGACACGTT | |
| Homo sapiens zinc finger protein 36, C3H type-like 1 (ZFP36L1) | F:5'-GGACTAGT AGGCCTTTCACAACTAGGACTGA |
| R:5'-CCCAAGCTT AAACTGCAAATAGTCGTTACAAA | |
| Homo sapiens transportin 1 | F:5'-GGACTAGT TCTAATACACTTAAGCTGCAGT |
| R:5'-CCCAAGCTT GCTTCTTCACATCCACTGCGGAGT | |
| Homo sapiens ribosomal protein L7a | F:5'-GGACTAGT GAAGACAAAGGCGCTTTGGCTA |
| R:5'-CCCAAGCTT ATGTACAGAAAACTCAACAGT | |
| Homo sapiens interleukin 32 | F:5'-GGACTAGT AGATACTGACACCACCTTTGCCCT |
| R:5'-CCCAAGCTT CATGGTATCTCCCCTGCCAG | |
| Homo sapiens mRNA for putative NFkB activating protein | F:5'-GGACTAGT TGAACACAGAAAGTCTAAGAGGA |
| R:5'-CCCAAGCTT GCTAATTAAACTTTGATTTTATTATG | |
| HCMV UL17/18 | F:5'-GGACTAGT TACCAGCGGTTACGCACCGAG |
| R:5'-CCCAAGCTT AACAGTTCCTCGGACATGATCA | |
| HCMV IE86 | F:5'-GGACTAGT AGTCCACGGACCGCTCGGTCT |
| R:5'-CCCAAGCTT TGCGCTCACCCGGCGTTCTC |