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Figure 1 | Virology Journal

Figure 1

From: A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

Figure 1

Schematic diagram of the transfer plasmid for construction of recombinant baculovirus. The structure of the transfer plasmid based on pFastBac Dual is displayed. pH, polyhedrin promoter of baculovirus; p10, p10 promoter of baculovirus; VSV/GED, truncated glycoprotein of vesicular stomatitis virus including 21 amino acid ectodomain with transmembrane and cytoplasmic tail domains; CMV, cytomegalovirus immediate-early promoter/enhancer; SV40 or tk poly (A), polyadenylation signal from Simian virus 40 or tk gene of Herpes simplex virus; P12A3C, the P12A3C gene encoding structural and non-structural proteins necessary for the formation of FMDV empty capsid; CAG, a composite promoter consisting of the CMV IE enhancer and chicken β-actin promoter; T-cell epitopes including a pan DR epitope sequence (PADRE), seven T cell epitope regions in structural or non-structural proteins of FMDV and a invasin immunostimulatory sequence taken from Yersinia, as described by Wang et al. (2002).

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Virology Journal

ISSN: 1743-422X