Masanori Terajima, University of Massachusetts Medical School
30 March 2012
I am surprised to see that ECV304 cell line is still being used as an endothelial cell line after two papers in 1999 and 2000 clearly showed that it is not.
The two papers are
1: Dirks WG, MacLeod RA, Drexler HG. ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross- contamination at source. In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):558-9. PubMed PMID: 10614862.
2: Brown J, Reading SJ, Jones S, Fitchett CJ, Howl J, Martin A, Longland CL, Michelangeli F, Dubrova YE, Brown CA. Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83. Lab Invest. 2000 Jan;80(1):37-45. PubMed PMID: 10653001.
Virology Journal
Is ECV304 an endothelial cell line?
30 March 2012
I am surprised to see that ECV304 cell line is still being used as an endothelial cell line after two papers in 1999 and 2000 clearly showed that it is not.
The two papers are
1: Dirks WG, MacLeod RA, Drexler HG. ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross- contamination at source. In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):558-9. PubMed PMID: 10614862.
2: Brown J, Reading SJ, Jones S, Fitchett CJ, Howl J, Martin A, Longland CL, Michelangeli F, Dubrova YE, Brown CA. Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83. Lab Invest. 2000 Jan;80(1):37-45. PubMed PMID: 10653001.
Competing interests
None declared