Highly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR

Table 2 Results of the ROC analysis for three separate dilution steps and the combined data set

	AUC	Cutpoint	Sensitivity	Specificity	Correctly classified	LR+	LR-	theta0	d0 (range)	upper limit (range)	lower limit (range)	VRP
Undiluted	0.9957	≥ 0.42	95.24%	100.00%	96.88%		0.0476	0.931	0.360 (0.283, 0.653)	0.416 (0.19, 0.416)	0.424 (0.283, 0.489)	1.003
1:10 diluted	0.9667	≥ 0.4551	90.00%	100.00%	93.75%		0.1	0.908	0.352 (3, 3)	0.328 (0.205, 0.328)	2.605 (3, 1.528)	1.764
1:100 diluted	0.9595	≥ 0.21	89.47%	100.00%	93.75%		0.1053	0.909	0.174 (3, 1.642)	0.162 (0.067, 0.162)	3.000 (3, 0.383)	1.946
Overall	0.9708	≥ 0.2122	95.00%	88.89%	92.71%	85.500	0.0562	0.917	0.264 (0.283, 0.416)	0.236 (0.09, 0.416)	0.571 (0.283, 1.017)	1.112

A non-parametric analysis was performed;
Abbreviations: Overall: all dilution steps were analysed together; AUC: area under the ROC-curve; LR+/LR-: Positive and negative likelihood ration; theta0/d0: co-ordinates at cut-off point of equivalence with specificity = sensitivity = theta0; IR: intermediate range, VRP: valid range proportion

ISSN: 1743-422X