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Table 1 Results of qPCR and ApoH-ELISA of samples and controls (undiluted, 1:10 or 1:100 dilution)

From: Highly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR

No.

Sample ID

Genotype

Undiluted sample

1:10 dilution in NaOAc-buffer (50 mM)

1:100 dilution in NaOAc-buffer (50 mM)

   

direct (qPCR) GE

ELISA OD

direct (qPCR) GE

ELISA 1:10 OD

direct (qPCR) GE

ELISA OD

1

09g1014

G1P[8]

8.9E+06

2.5224

2.4E+05

3.0000

4.4E+04

3.0000

2

09g429

G1P[8]

7.8E+06

3.0000

1.7E+07

2.2098

2.1E+06

1.2997

3

09g454

G1P[8]

6.5E+06

3.0000

8.6E+05

1.5300

1.0E+05

0.3800

4

09g517

G2P[4]

5.4E+05

3.0000

6.2E+04

3.0000

5.3E+03

1.6400

5

09g991

G2P[4]

8.8E+05

0.6528

1.6E+06

1.8972

7.7E+05

1.1210

6

09g928

G3P[8]

2.4E+08

2.1567

2.9E+07

1.7056

3.6E+06

0.3692

7

09g449

G4P[8]

3.0E+05

3.0000

5.3E+04

1.5100

9.7E+03

0.3900

8

09g451

G4P[8]

1.4E+06

1.0200

8.9E+04

0.7900

1.2E+04

0.2100

9

09g452

G4P[8]

3.5E+08

3.0000

6.7E+07

1.9517

4.3E+06

0.7855

10

09g453

G4P[8]

4.3E+08

3.0000

1.1E+08

1.4608

1.9E+07

0.6903

11

09g1022

G8P(4)

7.2E+07

1.1735

9.0E+06

0.9167

6.7E+05

0.3529

12

09g962

G9P[8]

1.2E+03

3.0000

310

3.0000

20

3.0000

13

09g103

G9P[8]

1.9E+08

1.3311

9.0E+07

1.8673

3.9E+04

2.3071

14

09g410

G9P[8]

3.8E+05

0.2800

5.2E+04

0.1200

4.2E+03

0.0400

15

09g1028

G12P[6]

1.1E+08

2.3188

5.6E+06

1.4600

6.6E+05

0.3582

16

RoA cc1

WA

7.6E+06

2.6300

9.8E+05

2.6900

1.5E+05

2.4100

17

RoA cc2

WA

2.3E+07

2.7957

2.3E+06

2.2964

2.4E+05

0.5170

18

RoA cc3

WA

1.8E+07

2.8673

1.9E+06

2.2590

1.8E+05

0.4448

19

HAV_1

n.s.

4

0.4887

n.a.

0.3276

n.a.

0.1622

20

HAV_2

n.s.

16

0.4200

50

0.2200

12

0.0900

21

Human2

n.a.

n.a.

0.2356

n.a.

0.3802

n.a.

0.1210

22

Human3

n.a.

n.a.

0.0981

n.a.

0.2047

n.a.

0.0666

23

Wild boar

n.a.

n.a.

0.0393

n.a.

0.0384

n.a.

0.0367

24

Rat

n.a.

n.a.

0.0903

n.a.

0.1205

n.a.

0.0189

25

Mouse

n.a.

n.a.

0.0443

n.a.

0.0304

n.a.

0.0229

26

Pig_1

n.a.

n.a.

0.4158

n.a.

0.1632

n.a.

0.0573

27

Pig_2

G4P[8]

135

0.8721

2

0.4551

n.a.

0.1841

28

FCS

n.a.

n.a.

0.0203

n.a.

0.0189

n.a.

0.0228

29

NaOAc1

n.a.

n.a.

0.0358

n.a.

0.0340

n.a.

0.0354

30

NaOAc2

n.a.

n.a.

0.0821

n.a.

0.0582

n.a.

0.0658

31

NaOAc3

n.a.

n.a.

0.1896

n.a.

0.1233

n.a.

0.0700

32

NaOAc4

n.a.

n.a.

0.0217

n.a.

0.0176

n.a.

0.0001

33

CC_dil-2

WA

10,000 IU

1.6464

-

-

-

-

34

CC_dil-3

WA

1,000 IU

0.3173

-

-

-

-

35

CC_dil-4

WA

100 IU

0.0550

-

-

-

-

36

CC_dil-5

WA

10 IU

0.0399

-

-

-

-

37

CC_dil-6

WA

1 IU

0.0297

-

-

-

-

38

CC_dil-7

WA

0.1 IU

0.0286

-

-

-

-

39

CC_dil-8

WA

0.01 IU

0.0315

-

-

-

-

  1. Abbreviations: IU: infectious units TCID50/ml measured in cell culture assay; GE: genome equivalents per reaction; OD: optical density at 450/620 nm; n.a.: no amplification; n.s.: no sequence result obtained; -: not done; CC_dil: dilution of cell culture virus stock
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