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Table 1 Results of qPCR and ApoH-ELISA of samples and controls (undiluted, 1:10 or 1:100 dilution)

From: Highly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR

No. Sample ID Genotype Undiluted sample 1:10 dilution in NaOAc-buffer (50 mM) 1:100 dilution in NaOAc-buffer (50 mM)
    direct (qPCR) GE ELISA OD direct (qPCR) GE ELISA 1:10 OD direct (qPCR) GE ELISA OD
1 09g1014 G1P[8] 8.9E+06 2.5224 2.4E+05 3.0000 4.4E+04 3.0000
2 09g429 G1P[8] 7.8E+06 3.0000 1.7E+07 2.2098 2.1E+06 1.2997
3 09g454 G1P[8] 6.5E+06 3.0000 8.6E+05 1.5300 1.0E+05 0.3800
4 09g517 G2P[4] 5.4E+05 3.0000 6.2E+04 3.0000 5.3E+03 1.6400
5 09g991 G2P[4] 8.8E+05 0.6528 1.6E+06 1.8972 7.7E+05 1.1210
6 09g928 G3P[8] 2.4E+08 2.1567 2.9E+07 1.7056 3.6E+06 0.3692
7 09g449 G4P[8] 3.0E+05 3.0000 5.3E+04 1.5100 9.7E+03 0.3900
8 09g451 G4P[8] 1.4E+06 1.0200 8.9E+04 0.7900 1.2E+04 0.2100
9 09g452 G4P[8] 3.5E+08 3.0000 6.7E+07 1.9517 4.3E+06 0.7855
10 09g453 G4P[8] 4.3E+08 3.0000 1.1E+08 1.4608 1.9E+07 0.6903
11 09g1022 G8P(4) 7.2E+07 1.1735 9.0E+06 0.9167 6.7E+05 0.3529
12 09g962 G9P[8] 1.2E+03 3.0000 310 3.0000 20 3.0000
13 09g103 G9P[8] 1.9E+08 1.3311 9.0E+07 1.8673 3.9E+04 2.3071
14 09g410 G9P[8] 3.8E+05 0.2800 5.2E+04 0.1200 4.2E+03 0.0400
15 09g1028 G12P[6] 1.1E+08 2.3188 5.6E+06 1.4600 6.6E+05 0.3582
16 RoA cc1 WA 7.6E+06 2.6300 9.8E+05 2.6900 1.5E+05 2.4100
17 RoA cc2 WA 2.3E+07 2.7957 2.3E+06 2.2964 2.4E+05 0.5170
18 RoA cc3 WA 1.8E+07 2.8673 1.9E+06 2.2590 1.8E+05 0.4448
19 HAV_1 n.s. 4 0.4887 n.a. 0.3276 n.a. 0.1622
20 HAV_2 n.s. 16 0.4200 50 0.2200 12 0.0900
21 Human2 n.a. n.a. 0.2356 n.a. 0.3802 n.a. 0.1210
22 Human3 n.a. n.a. 0.0981 n.a. 0.2047 n.a. 0.0666
23 Wild boar n.a. n.a. 0.0393 n.a. 0.0384 n.a. 0.0367
24 Rat n.a. n.a. 0.0903 n.a. 0.1205 n.a. 0.0189
25 Mouse n.a. n.a. 0.0443 n.a. 0.0304 n.a. 0.0229
26 Pig_1 n.a. n.a. 0.4158 n.a. 0.1632 n.a. 0.0573
27 Pig_2 G4P[8] 135 0.8721 2 0.4551 n.a. 0.1841
28 FCS n.a. n.a. 0.0203 n.a. 0.0189 n.a. 0.0228
29 NaOAc1 n.a. n.a. 0.0358 n.a. 0.0340 n.a. 0.0354
30 NaOAc2 n.a. n.a. 0.0821 n.a. 0.0582 n.a. 0.0658
31 NaOAc3 n.a. n.a. 0.1896 n.a. 0.1233 n.a. 0.0700
32 NaOAc4 n.a. n.a. 0.0217 n.a. 0.0176 n.a. 0.0001
33 CC_dil-2 WA 10,000 IU 1.6464 - - - -
34 CC_dil-3 WA 1,000 IU 0.3173 - - - -
35 CC_dil-4 WA 100 IU 0.0550 - - - -
36 CC_dil-5 WA 10 IU 0.0399 - - - -
37 CC_dil-6 WA 1 IU 0.0297 - - - -
38 CC_dil-7 WA 0.1 IU 0.0286 - - - -
39 CC_dil-8 WA 0.01 IU 0.0315 - - - -
  1. Abbreviations: IU: infectious units TCID50/ml measured in cell culture assay; GE: genome equivalents per reaction; OD: optical density at 450/620 nm; n.a.: no amplification; n.s.: no sequence result obtained; -: not done; CC_dil: dilution of cell culture virus stock