Figure 2

Screening of hMPV or hRSV-specific IgG positive and negative human sera samples. Examples of samples that are positive or negative for hMPV or hRSV. (A) Screening of hMPV positive and negative sera samples. Left, Western blot analysis was used to show the expression of hMPV N protein in positive sera (#221, #308, #471, #365, #199) and negative sera (#21, #75, #92, #99, #108). Right, two-fold serial dilutions of positive sera (#221, #308, #471, #365, #199) and negative sera (#21, #75, #92, #99, #108) were tested in plates coated with purified hMPV N protein as described in Methods section. (B) Screening of hRSV positive and hRSV negative sera samples. Left, Western blot analysis was used to show the expression of hRSV N protein in positive sera (#596, #112, #267, #126, #260) and negative sera (#34, #99, #100, #150, #207). Right, two-fold serial dilutions of positive sera (#596, #112, #267, #126, #260) and negative sera (#34, #99, #100, #150, #207) were tested in plates coated with purified hRSV N protein as described in Methods section. Arrows indicate positive bands.