Figure 5

Enterovirus 71 infection promotes degradation of TRIM38. (A) Detection of TRIM38 mRNA. A549 cells were mock-infected or infected with EV71 at 2 MOIs. At indicated time points, total RNA was extracted from cells. TRIM38 transcription level was analyzed by quantitative real-time PCR using SYBR-Green. GAPDH gene primer sets were used as an internal control. (B) Detection of TRIM38 protein. At 4, 8, 12, 24, 36 and 48 h post-infection of EV71, A549 cells were harvested and whole cell lysates were prepared for Western blot analysis with antibodies against TRIM38 and β-actin. (C) Detection of EV71 proteins. At 4, 8, 12, 24, 36 and 48 h post-infection of EV71, cells were subjected to In-cell Western blot analysis. Shown are representative images with the green color depicting EV71 proteins and the red color depicting lamin A, which was used as an internal control.