Figure 4
From: Enterovirus 71 induces degradation of TRIM38, a potential E3 ubiquitin ligase
TRIM38 catalyzes K63-linked ubiquitin to cellular proteins. (A) 293T cells were transfected with plasmid expressing Flag-TRIM38 (lanes 2), or Flag-ΔRING (lanes 3) and pRK5-HA-ubiquitin-K63-linked-Ubiquitin (lanes 1, 2, and 3). Whole cell lysates were subjected to Western blot analysis with antibodies against HA, Flag and β-actin. (B) Similar to (A), except that pRK5-HA-ubiquitin-K48-linked-Ubiquitin was used instead of pRK5-HA-ubiquitin-K63-linked-Ubiquitin.