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Table 1 Oligonucleotide sequences used for detection of HAdV

From: Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods

Primer

Sequence (5'→ 3')a

+/-b

Target

Ampliconsize (bp)

References

Q-Padv-F

AACGGCCGCTACTGCAAG

+

Swine AdV hexon

68

Hundesa et al., 2009 [24]

Q-Padv-R

AGCAGCAGGCTCTTGAGG

-

   

hex1deg (outer)

GCCSCARTGGKCWTACATGCACATC

+

Hexon

301

Allard et al., 2001 [25]

hex2deg (outer)

CAGCACSCCICGRATGTCAAA

-

   

nehex3deg (inner)

GCCCGYGCMACIGAIACSTACTTC

+

 

171

 

nehex4deg (inner)

CCYACRGCCAGIGTRWAICGMRCYTTGTA

-

   

ADV-F

GCCACGGTGGGGTTTCTAAACTT

+

Hexon

131

Gunson et al., 2009 [26]

ADV-R

GCCCCAGTGGTCTTACATGCACATC

-

   

XuHex1

TTCCCCATGGCICAYAACAC

+

Hexon

482

Xu et al. 2000 [27]

XuHex2

CCCTGGTAKCCRATRTTGTA

-

   

hexDEGF

CAGGACGCCTCGGRGTAYCTSAG

+

Hexon

103

Damen et al., 2008 [28]

hexDEGR

GGAGCCACVGTGGGRTT

-

   

AdE1

TCCCTACGATGCAGACAACG

+

Fiber

967

Xu et al. 2000 [27]

AdE2

AGTGCCATCTATGCTATCTCC

-

   

AdF1

ACTTAATGCTGACACGGGCAC

+

 

541-586

 

AdF2

TAATGTTTGTGTTACTCCGCTC

-

   

AdF

CWTACATGCACATCKCSGG

+

Hexon

~75

Hernroth et al., 2002 [29]

AdR

CRCGGGCRAAYTGCACCAG

-

   

HAdV-ABCDEF-hexon25fc

CARTGGKCDTACATGCACATC

+

Hexon

 

Kuo et al., 2009 [30]

HAdV-E-hexon373r

CCAGRCTGTTGTAGGCAGTG

-

 

349

 

HAdV-F-hexon265r

CCACGGCCAGCGTAAAGC

-

 

241

 

hexAA1885 (outer)

GCCGCAGTGGTCTTACATGCACAGC

+

Hexon

300

Allard et al., 1990 [31]

hexAA1913 (outer)

CAGCACGCCGCGGATGTCAAAGT

-

   

nehexAA1893 (inner)

GCCACCGAGACGTACTTCAGCCTG

+

Hexon

142

Allard et al., 1992 [32]

nehexAA1905 (inner)

TTGTACGAGTACGCGGTATCCTCGCGGTC

-

   

JTVFF

AACTTTCTCTCTTAATAGACGCC

+

Fiber

117

Jothikumar et al., 2005 [33]

JTVFR

AGGGGGCTAGAAAACAAAA

-

   

AdV1 (outer)

CAAGATGGCCACCCCCTCG

+

hexon

329

Oh et al., 2003 [34]

AdV2 (outer)

CGATCCAGCACGCCGCGGATGTC

-

   

AdV3 (inner)

AATGGTCTTACATGCACAT

+

 

253

 

AdV4 (inner)

ACCCGGTTGTCGCCCACGGCCAG

-

   
  1. a. R = A+G; Y = C+T;S = C+G;W = A+T;H = A+C+T;B = C+G+T;V = A+C+G;D = A+G+T;N = all
  2. b. Polarity
  3. c. Forward primer for both HAdV-E-hexon373r and HAdV-F-hexon265r
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Virology Journal

ISSN: 1743-422X