Figure 7

The CD4⁺ and CD8⁺ T lymphocyte subsets in the different groups were assessed by FACS analysis at day 10 post the final boost immunization. Spleen lymphocytes of mice in different immunized groups were repectively enriched from the spleens at days 10 post the final boost immunization and cultured in RPMI 1640 medium. The CD4⁺ and CD8⁺ T lymphocyte subsets in the different groups were assessed by FACS in order to examine whether or not DNA vaccine constructs immunized mice by skin scarification can induce a T-cell response in mice and further activate cell immunity response. (A) PBS; (B) pcDNA3.1(+) vector; (C) pcDNA3.1-ORFV011; (D) pcDNA3.1-ORFV059; (E) pcDNA3.1-ORFV011 plus pcDNA3.1-ORFV059; (F) pcDNA3.1-ORFV011/ORFV059.