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Figure 2 | Virology Journal

Figure 2

From: Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants

Figure 2

VIGS reduction of NbRBR1 and NbPCNA expression (a) Semi-quantitative reverse transcription PCR (sqRT-PCR) assays of NbRBR1 mRNA levels in transgenic Nicotinana benthamiana line pOri-2 plants inoculated with PVX/GFP (GFP) or PVX/NbRBR1-GFP (NbRBR1-GFP, P1-P4 for four individual plants). All plants were at the age of 56 days. (b) SqRT-PCR assays of NbPCNA mRNA levels in healthy pOri2 plant (mock), or plants inoculated with PVX/GFP (GFP), PVX/NbRRB1-GFP (NbRBR1-GFP), or PVX/NbPCNA-GFP (NbPCNA-GFP). SqRT-PCR of 18S rRNA indicates similar amount of total RNAs used in each sample. The positions and sizes of 1-kb DNA marker (Ladder) and positions of RT-PCR products specific for NbRBR1 and NbPCNA mRNA as well as 18S rRNA are indicated.

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Virology Journal

ISSN: 1743-422X