Table 3 Quantitative PCR of the miRNA-targeted gag gene inhibiting ALV-J
From: Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs
Group | mRNA levels of gag | mRNA levels of β-actin | mRNA levels of target gene by β-actin normalization | Rates of viral suppression (%) | p-values |
|---|---|---|---|---|---|
mi-gag 1318 | 1.05 ± 0.11 | 3.21 ± 0.12 | 0.327 | 77.3* | 0.01 |
mi-gag 1365 | 1.10 ± 0.12 | 3.14 ± 0.15 | 0.3350 | 65.0* | 0.02 |
mi-gag 1623 | 2.81 ± 0.11 | 3.49 ± 0.16 | 0.805 | 19.5 | 0.06 |
mi-gag 1971 | 3.12 ± 0.17 | 3.85 ± 0.16 | 0.810 | 19.0 | 0.07 |
Negative control | 3.15 ± 0.12 | 3.14 ± 0.12 | 1. 00 | 0 | 0 |
Vector control | 3.62 ± 0.14 | 3.70 ± 0.12 | 0.978 | 0 | 0 |