Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

Table 1 Primers used for RT-LAMP and RT-PCR

	Primer	Type	Target gene	Sequence(5'-3')	Genome position^a
	F3-1	Forward outer		TGAAACACGGCGCATCTG	665-682
I	B3-1	Reverse outer	CP	AGTTCTGGGTGTCCATCAGT	875-856
	FIP-1	Forward inner		GCAACTTCGATGTCCTGTGGGTCCGCGTACGCTTTTGACT	752-731,689-706
	BIP-1	Reverse inner		AGCACGTCTTGCTGCTTTAGGCGGTTGTCTTGCGGAGGTT	759-780,837-820
	F3-2	Forward outer		GCAGATCGTGTTGAGGCC	106-123
II	B3-2	Reverse outer	CP	ATAGATTTGGGTGCGCTCTT	332-313
	FIP-2	Forward inner		CCAGCGTCGGCAACAATTTTGCAGCGATGACAAGAAAGCCAG	212-191,142-161
	BIP-2	Reverse inner		CAAAAAGGACCAATGCCGCCACGAAGCTTTAGTCCTGCGTT	215-235,293-274
	F3-3	Forward outer		ACTACCATTCTCGCAGCACT	715-734
III	B3-3	Reverse outer	72 kDa	GGTCACGAACGACAGTGC	916-899
	FIP-3	Forward inner		GCCGAACCATGGAAAGGTGTCT-TGGCGTTGTTTCCTTAGTGC	813-729,748-767
	BIP-3	Reverse inner		TACTCCCACGGAGGTCCTCC-GCTGGTAAGAAGCATCCCG	814-833,889-871
	F3-4	Forward outer		GGGGTTTCGAACACGAATGG	1335-1354
IV	B3-4	Reverse outer	72 kDa	AGCGAGGCCTCAGCATTA	1517-1500
	FIP-4	Forward inner		CATGTCCAGTGCTCTTGCGTCG-CATCCACGCTGCAACGAA	1428-1407,1355-1372
	BIP-V	Reverse inner		ACGAGCGAGCTTTTCCAATCCA-CAAGCAGCGAGACAATGTCA	1429-1450,1498-1479
	HC72-157 F	Forward Primer	72 kDa	TGCGAAGCCTCTACGACCTGTTT	157-179
	HC72-518R	Reverse Primer		TGATGATTGCTCGCCCAACAGA	518-497
	CWMV-F	Forward Primer	19 kDa	TATGACTACTGGTACTCA	2652-2669
	CWMV-R	Reverse Primer		AATTACTCCACACGAGT	3173-3157
	BSMV-F	Forward Primer	unknown	CTTGATGCTTTGGATAAGGCTTA	1864-1887
	BSMV-R	Reverse Primer	protein	AATCTTCCCTTGGGGGAC	2790-2773

^a Genome position refers to the nucleotide sequence of WYMV-HC CP and 72 kDa genes (accession number RNA1 AF067124 and RNA2 AF041041), CWMV RNA2 19-kDa gene (accession number AJ012006), and BSMV RNA-gamma segment (accession number M16577)

ISSN: 1743-422X