Figure 2

Phylogenetic analysis of 24 VZV strains. Nucleotide or amino acid sequences were multiple-aligned using ClustalW program (ver 2.0.1) and the resulting *.phy files were used to construct phylogenetic trees using maximum-likelihood (ML) or neighbor-joining (NJ) methods in Phylip package (version 3.69). (a) ML tree based on full nucleotide sequences. (b) ML tree based on non-coding sequences. (c) NJ tree based on the nucleotide sequences of ORF62, showing clear separation of vaccine strains from pOka within clade 2. (d) NJ tree based on the nucleotide sequences of ORF1. Vaccine strains are separated from clinical strains, but formation of clade 2 is not evident.