Figure 3

Inhibition of RD-114 production by overexpression of the dominant-negative mutants of Vps4A/B. (A) 293 T cells were cotransfected with pTERD-114 (100 ng) and either the expression plasmids for Vps4A/B dominant-negative mutant containing N-terminal FLAG-tag, or the empty vector pCDNFL (Control) (100 ng), and then analyzed by Western blotting (upper) and real-time RT-PCR (lower) as in Figure 1. The virus production in Control was set to 100%. The data are shown as averages and standard deviations of 3 independent experiments. Statistical analysis was performed by using the Student's t-test (*: p < 0.01). (B) CRFK cells were transfected with the expression plasmids Vps4A/B dominant-negative mutant (1 μg). Cells and viruses were collected at 72 h after transfection, and analyzed by Western blotting (upper) and real-time RT-PCR (lower) as in (A).